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Instrumentation
Mass Spectrometers:
| ThermoElectron LTQ-Orbitrap - nanospray infusion or LC/MS or LC/MSMS of peptides and Proteins with CID or ETD |
| ThermoElectron LTQ-FTICR - nanospray infusion or on-line LC/MS with CID, IRMPD, or ECD |
| ABI 4700 MALDI-TOF-TOF - automated MS and MS/MS analysis of peptides |
| MDS Sciex/ABI QSTAR Elite Q-TOF - LC/MSMS of peptide digests with iTRAQ/SILAC quantitation |
| MDS Sciex/ABI QSTAR XL Q-TOF - LC/MS analysis of proteins and peptides |
| MDS Sciex/ABI QSTAR Pulsar Q-TOF - LC/MS analysis of proteins and peptides |
| *PE-Biosystems Mariner ESI-TOF - accurate mass measurement of small molecules |
| *PE-Biosystems Voyager Elite STR MALDI-TOF - linear or reflectron MS of biomolecules |
| *Sciex API 300 triple quadrupole - low resolution infusion or LC/MS |
* open for training for outside users within UCSF
Off-line Chromatography
| Amersham Biosciences Akta HPLC system - Large scale sample preparation using ion exchange/size exclusion/affinity/hydrophilic interaction/reversed phase chromaography |
| Beckman Gold HPLC system - Reversed phase sample preparation |
| Ultimate HPLC system with Probot (DionexLC Packings) - Reversed phase sample separation for LC-MALDI |
On-line nanoLC-MS
| Waters NanoAcuity LC system |
| Famos autosampler/Ultimate HPLC system(Dionex/LC Packings) |
| Spark autosampler/Eksigent nanopump |
| Spark autosampler/Eldex syringe pump |
| Agilent Technologies 1100 series nanoHPLC system |
The UCSF Mass Spectrometry Facility (A.L. Burlingame, Director) is supported by the Biomedical Technology Resource Centers Program of the National Center for Research Resources, NIH NCRR RR01614